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Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients BJID
Debur,Maria do Carmo; Vidal,Luine R. Rosele; Nogueira,Meri Bordignon; Pereira,Luciane Aparecida; Takahashi,Gislene R.A.; Rotta,Indianara; Almeida,Sérgio Monteiro de; Raboni,Sonia Mara.
We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunoperoxidase; Immunofluorescence; Cytomegalovirus; Immunocompromised patients; Bone marrow transplantation; Solid organ transplant.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200014
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Pathologic study of a fatal case of dengue-3 virus infection in Rio de Janeiro, Brazil BJID
Basílio-de-Oliveira,C.A.; Aguiar,G.R.; Baldanza,M.S.; Barth,O.M.; Eyer-Silva,W.A.; Paes,M.V..
Dengue hemorrhagic fever (DHF) is a severe febrile disease, characterized by abnormalities in hemostasis and increased vascular permeability, which in some cases results in hypovolemic shock syndrome and in dengue shock syndrome. The clinical features of DHF include plasma leakage, bleeding tendency and liver involvement. We studied the histopathological features of a fatal case of dengue-3 virus infection. The patient, a 63-year old male, presented with an acute onset of severe headache, myalgia and maculopapular rash. Tissue fragments (liver, spleen, lung, heart, kidney and lymph nodes) were collected for light microscopy studies and stained by standard methods. Histopathology revealed severe tissue damage, caused by intense hemorrhage, interstitial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue virus serotype 3; Dengue hemorrhagic fever; Immunoperoxidase.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000400012
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Potentially pathogenic mycoplasmas in the external ear canal of clinically normal cattle in Southeast Brazil: first report BJM
Santos,Sandra Batista dos; Nascimento,Elmiro Rosendo do; Faccini,João Luiz Horácio; Barreto,Maria Lúcia; Pereira,Virginia Léo de Almeida.
Mycoplasmas were searched in the ear canal flushing of 60 bovine in Brazil. The prevalence obtained was 80%. The percentages of typified species were 12.5%, for M. alkalenses; 2.1%, M. arginini; 8.35%, M. bovirhinis; 2.1%, M. bovis; 25.0%, M. conjunctivae; 14.6%, M. mycoides subsp. mycoides LC and 10.4% M. capricolum.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Bovine; External ear canal; Immunoperoxidase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300006
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